This study was carried out to develop an effective liquid-phase double antibody enzyme immunoassay for determining of progesterone.
The optimum conditions of assay system, lst and 2nd antibodies, enzyme conjugate, and time reaction were invested.
The bovine plasma progesterone level in dairy cattle and korean native bulls were also analyzed.
The results obtained were as follows;
1. The reproducibility of petroleum ether was superior to Ihat of ethyl ether as extract solvent of progesterone in plasma
2. The optimum dilution rate of lst and 2nd antibody was 30,000 and 10 times, respectively. Affer the reaction of enzyme conjugate to progesterone lst antibody, and then 2nd antibody competition reaction was enough for over 1hr.
3. Average plasma progesterone level in 4 pregnant and 9 nonpregnant Holstein was 2.5±0.5 and 0.7±0.2ng/㎖, respectively. Average plasma progesterone level of 10 Korean native bulls was 0.1±0.001ng/㎖
From these results, by using liquid phase double antibody enzyme immunoassay for progesterone is applicable to detect of early pregnancy diagnosis, factorial analysis of reproductive disorder, and also reproductive physiological function such as monitoring of cyclicity during the post-partum period.
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